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  Functional Genomic Analysis of Two Staphylococcus aureus Phages Isolated from the Dairy Environment.

García, P., Martínez, B., Obeso, J. M., Lavigne, R., Lurz, R., & Rodríguez, A. (2009). Functional Genomic Analysis of Two Staphylococcus aureus Phages Isolated from the Dairy Environment. Applied and Environmental Microbiology, 75(24), 7663-7673. doi:10.1128/AEM.01864-09.

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Genre: Zeitschriftenartikel
Alternativer Titel : Appl. Environ. Microbiol.

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 Urheber:
García, Pilar, Autor
Martínez, Beatriz, Autor
Obeso, José María, Autor
Lavigne, Rob, Autor
Lurz, Rudi1, Autor
Rodríguez, Ana, Autor
Affiliations:
1Max Planck Society, ou_persistent13              

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 Zusammenfassung: The genomes of the two lytic mutant Staphylococcus aureus bacteriophages, vB_SauS-phiIPLA35 (phiIPLA35) and vB_SauS-phiIPLA88 (phiIPLA88), isolated from milk have been analyzed. Their genomes are 45,344 bp and 42,526 bp long, respectively, and contain 62 and 61 open reading frames (ORFS). Enzymatic analyses and sequencing revealed that the phiIPLA35 DNA molecule has 3'-protruding cohesive ends (cos) 10 bp long, whereas phiIPLA88 DNA is 4.5% terminally redundant and most likely is packaged by a headful mechanism. N-terminal amino acid sequencing, mass spectrometry, bioinformatic analyses, and functional analyses enabled the assignment of putative functions to 58 gene products, including DNA packaging proteins, morphogenetic proteins, lysis components, and proteins necessary for DNA recombination, modification, and replication. Point mutations in their lysogeny control-associated genes explain their strictly lytic behavior. Muralytic activity associated with other structural components has been detected in virions of both phages. Comparative analysis of phiIPLA35 and phiIPLA88 genome structures shows that they resemble those of {phi}12 and {phi}11, respectively, both representatives of large genomic groupings within the S. aureus-infecting phages.

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Sprache(n): eng - English
 Datum: 2009-12-01
 Publikationsstatus: Erschienen
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Titel: Applied and Environmental Microbiology
  Alternativer Titel : Appl. Environ. Microbiol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 75 (24) Artikelnummer: - Start- / Endseite: 7663 - 7673 Identifikator: ISSN: 0099-2240