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  A streamlined protocol for emulsion polymerase chain reaction and subsequent purification.

Schütze, T., Rubelt, F., Repkow, J., Greiner, N., Erdmann, V. A., Lehrach, H., et al. (2010). A streamlined protocol for emulsion polymerase chain reaction and subsequent purification. Analytical Biochemistry, 410(1), 155-157. doi:10.1016/j.ab.2010.11.029.

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Genre: Journal Article
Alternative Title : Anal Biochem

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Schütze_sdarticle.pdf (Any fulltext), 468KB
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 Creators:
Schütze, T.1, Author           
Rubelt, F.2, Author           
Repkow, J.1, Author           
Greiner, N.1, Author           
Erdmann, V. A., Author
Lehrach, H.1, Author           
Konthur, Z.2, Author           
Glokler, J.1, Author           
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479653              

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 Abstract: Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications.

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Language(s): eng - English
 Dates: 2010-03-01
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: eDoc: 553212
DOI: 10.1016/j.ab.2010.11.029
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Title: Analytical Biochemistry
  Alternative Title : Anal Biochem
Source Genre: Journal
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Pages: - Volume / Issue: 410 (1) Sequence Number: - Start / End Page: 155 - 157 Identifier: ISSN: 0003-2697