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  The primary transcriptome of the major human pathogen Helicobacter pylori

Sharma, C. M., Hoffmann, S., Darfeuille, F., Reignier, J., Findeiß, S., Sittka, A., et al. (2010). The primary transcriptome of the major human pathogen Helicobacter pylori. Nature, 464(7286), 250-255. doi:10.1038/nature08756.

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Genre: Journal Article
Alternative Title : Nature

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 Creators:
Sharma, Cynthia M.1, Author
Hoffmann, Steve, Author
Darfeuille, Fabien, Author
Reignier, Jérémy, Author
Findeiß, Sven, Author
Sittka, Alexandra1, Author
Chabas, Sandrine, Author
Reiche, Kristin, Author
Hackermüller, Jörg, Author
Reinhardt, Richard2, Author           
Stadler, Peter F.1, Author
Vogel, Jörg1, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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 Abstract: Genome sequencing of Helicobacter pylori has revealed the potential proteins and genetic diversity of this prevalent human pathogen, yet little is known about its transcriptional organization and noncoding RNA output. Massively parallel cDNA sequencing (RNA-seq) has been revolutionizing global transcriptomic analysis. Here, using a novel differential approach (dRNA-seq) selective for the 5′ end of primary transcripts, we present a genome-wide map of H. pylori transcriptional start sites and operons. We discovered hundreds of transcriptional start sites within operons, and opposite to annotated genes, indicating that complexity of gene expression from the small H. pylori genome is increased by uncoupling of polycistrons and by genome-wide antisense transcription. We also discovered an unexpected number of ~60 small RNAs including the ϵ-subdivision counterpart of the regulatory 6S RNA and associated RNA products, and potential regulators of cis- and trans-encoded target messenger RNAs. Our approach establishes a paradigm for mapping and annotating the primary transcriptomes of many living species.

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Language(s): eng - English
 Dates: 2010-03-11
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature
  Alternative Title : Nature
Source Genre: Journal
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Pages: - Volume / Issue: 464 (7286) Sequence Number: - Start / End Page: 250 - 255 Identifier: ISSN: 0028-0836