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  Localization of eukaryote-specific ribosomal proteins in a 5.5-A cryo-EM map of the 80S eukaryotic ribosome

Armache, J. P., Jarasch, A., Anger, A. M., Villa, E., Becker, T., Bhushan, S., et al. (2010). Localization of eukaryote-specific ribosomal proteins in a 5.5-A cryo-EM map of the 80S eukaryotic ribosome. Proceedings of the National Academy of Sciences USA, 107(46), 19754-19759. doi:10.1073/pnas.1010005107.

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Genre: Journal Article
Alternative Title : Proc Natl Acad Sci USA

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 Creators:
Armache, J. P., Author
Jarasch, A., Author
Anger, A. M., Author
Villa, E., Author
Becker, T., Author
Bhushan, S., Author
Jossinet, F., Author
Habeck, M., Author
Dindar, G., Author
Franckenberg, S., Author
Marquez, V., Author
Mielke, T.1, Author           
Thomm, M., Author
Berninghausen, O., Author
Beatrix, B., Author
Soding, J., Author
Westhof, E., Author
Wilson, D. N.2, Author           
Beckmann, R., Author
Affiliations:
1Imaging/Electron Microscopy (Head: Rudi Lurz/Thorsten Mielke), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Cryoelectron Microscopy; Eukaryotic Cells/metabolism/ultrastructure; Evolution, Molecular; Models, Molecular; Protein Transport; RNA, Ribosomal/chemistry/genetics/ultrastructure; Ribosomal Proteins/metabolism/ultrastructure; Ribosomes/metabolism/ultrastructure; Saccharomyces cerevisiae/metabolism/ultrastructure; Species Specificity; Triticum/metabolism
 Abstract: Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-A resolution, together with a 6.1-A map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.

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Language(s): eng - English
 Dates: 2010-11-16
 Publication Status: Issued
 Pages: -
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 Identifiers: eDoc: 541908
DOI: 10.1073/pnas.1010005107
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Title: Proceedings of the National Academy of Sciences USA
  Alternative Title : Proc Natl Acad Sci USA
Source Genre: Journal
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Pages: - Volume / Issue: 107 (46) Sequence Number: - Start / End Page: 19754 - 19759 Identifier: ISSN: 1091-6490 (Electronic)