English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  The organization of the osteocyte network mirrors the extracellular matrix orientation in bone

Kerschnitzki, M., Wagermaier, W., Roschger, P., Seto, J., Shahar, R., Duda, G. N., et al. (2011). The organization of the osteocyte network mirrors the extracellular matrix orientation in bone. J Struct Biol, 173(2), 303-11. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21081167 http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=272607&_user=28761&_pii=S1047847710003515&_check=y&_origin=article&_zone=toolbar&_coverDate=28-Feb-2011&view=c&originContentFamily=serial&wchp=dGLzVlV-zSkzV&md5=e25715008c0af23c34cd2a6a712b6f23/1-s2.0-S1047847710003515-main.pdf.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Kerschnitzki, M., Author
Wagermaier, W., Author
Roschger, P., Author
Seto, J., Author
Shahar, R., Author
Duda, G. N., Author
Mundlos, S.1, Author           
Fratzl, P., Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

Content

show
hide
Free keywords: Animals; Cattle; Extracellular Matrix/*metabolism/*ultrastructure; Horses; Mice; Microscopy, Confocal; Microscopy, Electron, Scanning; Osteocytes/*metabolism/*ultrastructure; Sheep
 Abstract: Bone is a dynamic tissue that is continually undergoing a process of remodeling - an effect due to the interplay between bone resorption by osteoclasts and bone formation by osteoblasts. When new bone is deposited, some of the osteoblasts are embedded in the mineralizing collagen matrix and differentiate to osteocytes, forming a dense network throughout the whole bone tissue. Here, we investigate the extent to which the organization of the osteocyte network controls the collagen matrix arrangement found in various bone tissues. Several tissue types from equine, ovine and murine bone have been examined using confocal laser scanning microscopy as well as polarized light microscopy and back-scattered electron imaging. From comparing the spatial arrangements of unorganized and organized bone, we propose that the formation of a highly oriented collagen matrix requires an alignment of osteoblasts whereby a substrate layer provides a surface such that osteoblasts can align and, collectively, build new matrix. Without such a substrate, osteoblasts act isolated and only form matrices without long range order. Hence, we conclude that osteoblasts synthesize and utilize scaffold-like primary tissue as a guide for the deposition of highly ordered and mechanically competent bone tissue by a collective action of many cells.

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: J Struct Biol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 173 (2) Sequence Number: - Start / End Page: 303 - 11 Identifier: ISSN: 1095-8657 (Electronic) 1047-8477 (Linking)