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  A BACH2-BCL2L1 fusion gene resulting from a t(6;20)(q15;q11.2) chromosomal translocation in the lymphoma cell line BLUE-1

Turkmen, S., Riehn, M., Klopocki, E., Molkentin, M., Reinhardt, R., & Burmeister, T. (2011). A BACH2-BCL2L1 fusion gene resulting from a t(6;20)(q15;q11.2) chromosomal translocation in the lymphoma cell line BLUE-1. Genes Chromosomes Cancer, 50(6), 389-96. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21412927 http://onlinelibrary.wiley.com/store/10.1002/gcc.20863/asset/20863_ftp.pdf?v=1&t=gyzw8vx9&s=d7a278cbc50ee7f48bdf3f0dd1c5fcba6b1e3e4e.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-77FA-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-77FB-4
Genre: Journal Article

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Turkmen, S.1, Author              
Riehn, M., Author
Klopocki, E.2, Author              
Molkentin, M., Author
Reinhardt, R.3, Author              
Burmeister, T., Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433549              
2Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433557              
3High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433552              

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Free keywords: Basic-Leucine Zipper Transcription Factors/*genetics; Burkitt Lymphoma/*genetics; Cell Line, Tumor; Chromosomes, Human, Pair 20/*genetics; Chromosomes, Human, Pair 6/*genetics; Humans; Oncogene Proteins, Fusion/*genetics; Translocation, Genetic/*genetics; bcl-X Protein/*genetics
 Abstract: Abnormalities of the long arm of chromosome 6 are a common feature in various B-cell malignancies. In most cases, the genes involved have not yet been clearly identified. We have molecularly characterized the recently established Burkitt lymphoma cell line BLUE-1 that carries a t(6;20)(q15;q11.2) rearrangement in addition to the typical t(8;14) with MYC-IGH fusion. To identify the gene loci involved on both chromosomes we applied a sequential BAC clone mapping strategy. By using RT-PCR we were finally able to detect a chimeric mRNA transcript showing a fusion of the first (non-coding) exon of BACH2 (BTB and CNC homology 1, basic leucine zipper transcription factor 2) on 6q15 to the second exon of BCL2L1 (BCL-X) on 20q11. Various fusion transcripts were detected for different BCL2L1 (BCL-XL) isoforms. The fusion ultimately results in strong expression of the BCL2L1 (BCL-XL) anti-apoptosis protein, as demonstrated by immunoblotting. This is the first report that shows the involvement of both BCL2L1 and the transcription factor BACH2 in a chromosomal rearrangement. It points to BACH2 as a possibly important target in lymphomas with 6q aberrations, although other genes on 6q are probably also involved in these cases. Moreover, it suggests that members of the BCL2 anti-apoptosis gene family other than BCL2 itself might also be involved in lymphoma.

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 Dates: 2011
 Publication Status: Published in print
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Title: Genes Chromosomes Cancer
Source Genre: Journal
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Pages: - Volume / Issue: 50 (6) Sequence Number: - Start / End Page: 389 - 96 Identifier: ISSN: 1098-2264 (Electronic) 1045-2257 (Linking)