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  Sic1 plays a role in timing and oscillatory behaviour of B-type cyclins

Barberis, M., Linke, C., Adrover, M. A., Gonzalez-Novo, A., Lehrach, H., Krobitsch, S., et al. (2012). Sic1 plays a role in timing and oscillatory behaviour of B-type cyclins. Biotechnol Adv, 30(1), 108-130. doi:10.1016/j.biotechadv.2011.09.004.

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Barberis, M.1, Autor           
Linke, C.2, Autor           
Adrover, M. A., Autor
Gonzalez-Novo, A., Autor
Lehrach, H.3, Autor           
Krobitsch, S.2, Autor           
Posas, F., Autor
Klipp, E.1, Autor           
Affiliations:
1Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433554              
2Neurodegenerative Disorders (Sylvia Krobitsch), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479661              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Schlagwörter: Cell cycle; Oscillations; Cyclin waves; B-type cyclins; Sic1; Budding yeast
 Zusammenfassung: Budding yeast cell cycle oscillates between states of low and high cyclin-dependent kinase activity, driven by association of Cdk1 with B-type (Clb) cyclins. Various Cdk1-Clb complexes are activated and inactivated in a fixed, temporally regulated sequence, inducing the behaviour known as "waves of cyclins". The transition from low to high Clb activity is triggered by degradation of Sic1, the inhibitor of Cdk1-Clb complexes, at the entry to S phase. The G(1) phase is characterized by low Clb activity and high Sic1 levels. High Clb activity and Sic1 proteolysis are found from the beginning of the S phase until the end of mitosis. The mechanism regulating the appearance on schedule of Cdk1-Clb complexes is currently unknown. Here, we analyse oscillations of Clbs, focusing on the role of their inhibitor Sic1. We compare mathematical networks differing in interactions that Sic1 may establish with Cdk1-Clb complexes. Our analysis suggests that the wave-like cyclins pattern derives from the binding of Sic1 to all Clb pairs rather than from Clb degradation. These predictions are experimentally validated, showing that Sic1 indeed interacts and coexists in time with Clbs. Intriguingly, a sic1Delta strain looses cell cycle-regulated periodicity of Clbs, which is observed in the wild type, whether a SIC1-0P strain delays the formation of Clb waves. Our results highlight an additional role for Sic1 in regulating Cdk1-Clb complexes, coordinating their appearance.

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 Datum: 2011-09-182012-01
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/j.biotechadv.2011.09.004
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Titel: Biotechnol Adv
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 30 (1) Artikelnummer: - Start- / Endseite: 108 - 130 Identifikator: ISSN: 1873-1899 (Electronic) 0734-9750 (Print)