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Free keywords:
Amino Acid Sequence; *Bacterial Proteins/ge [Genetics]; Base Sequence; Carbohydrates/an [Analysis]; Fatty Acids/an [Analysis]; *Genes, Bacterial; *Genes, Structural; *Gram-Positive Bacteria/ge [Genetics]; Membrane Lipids/an [Analysis]; Molecular Sequence Data; Protein Processing, Post-Translational; Support, Non-U.S. Gov't
Abstract:
The complete nucleotide sequence of the gene encoding the surface (hexagonally packed intermediate [HPI])-layer polypeptide of Deinococcus radiodurans Sark was determined and found to encode a polypeptide of 1,036 amino acids. Amino acid sequence analysis of about 30% of the residues revealed that the mature polypeptide consists of at least 978 amino acids. The N terminus was blocked to Edman degradation. The results of proteolytic modification of the HPI layer in situ and Mr estimations of the HPI polypeptide expressed in Escherichia coli indicated that there is a leader sequence. The N-terminal region contained a very high percentage (29%) of threonine and serine, including a cluster of nine consecutive serine or threonine residues, whereas a stretch near the C terminus was extremely rich in aromatic amino acids (29%). The protein contained at least two disulfide bridges, as well as tightly bound reducing sugars and fatty acids.
