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Free keywords:
*Bacterial Proteins/ul [Ultrastructure]; *Collagen/ul [Ultrastructure]; Crystallization; *Gram-Positive Bacteria/an [Analysis]; Membrane Proteins/ul [Ultrastructure]; *Microscopy, Electron, Scanning; Support, Non-U.S. Gov't
Abstract:
When imaging biomacromolecules with a STM, coating of specimens with a conductive layer is a convenient preparation method which provides a good rate of success. Utilizing evaporated platinum/carbon as a coating film we have investigated two biomacromolecules of very different appearance. The first of these is the HPI-layer, a natural two-dimensional protein crystal with a period of 18 nm, which is found on the surface of the bacterium Deinococcus radiodurans. The second specimen is type IV collagen which forms long triple-helical strands approx. 1.5 nm in diameter. The resulting STM pictures compare very well with electron microscopical images.
