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  Thickness determination of biological samples with a z-calibrated scanning tunneling microscope

Wang, Z. H., Hartmann, T., Baumeister, W., & Guckenberger, R. (1990). Thickness determination of biological samples with a z-calibrated scanning tunneling microscope. Proceedings of the National Academy of Sciences of the United States of America., 87(23), 9343-9347.

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Wang, Z. H., Author
Hartmann, T., Author
Baumeister, W.1, Author           
Guckenberger, R.1, Author           
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1External Organizations, ou_persistent22              

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Free keywords: *Bacteriorhodopsins/ul [Ultrastructure]; Gold; Gram-Positive Bacteria/me [Metabolism]; Halobacterium/me [Metabolism]; Indicators and Reagents; *Microscopy, Scanning Tunneling/mt [Methods]; Support, Non-U.S. Gov't
 Abstract: A single-tube scanning tunneling microscope has been zeta-calibrated by using atomic steps of crystalline gold and was used for measuring the thickness of two biological samples, metal-coated as well as uncoated. The hexagonal surface layer of the bacterium Deinococcus radiodurans with an open network-type structure shows thickness values that are strongly influenced by the substrate and the preparation method. In contrast, the thickness of the purple membrane of Halobacterium halobium with its densely packed less-corrugated structure exhibits very little variation in thickness in coated preparations and the values obtained are in good agreement with x-ray data.

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 Dates: 1990
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: eDoc: 318491
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Title: Proceedings of the National Academy of Sciences of the United States of America.
Source Genre: Journal
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Pages: - Volume / Issue: 87 (23) Sequence Number: - Start / End Page: 9343 - 9347 Identifier: -