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  Electron microscopy and image analysis reveal common principles of organization in two large protein complexes: groEL-type proteins and proteasomes

Zwickl, P., Pfeifer, G., Lottspeich, F., Kopp, F., Dahlmann, B., & Baumeister, W. (1990). Electron microscopy and image analysis reveal common principles of organization in two large protein complexes: groEL-type proteins and proteasomes. Journal of Structural Biology., 103(3), 197-203.

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Zwickl, P.1, Author           
Pfeifer, G.1, Author           
Lottspeich, F.1, Author           
Kopp, F., Author
Dahlmann, B., Author
Baumeister, W.1, Author           
Affiliations:
1External Organizations, ou_persistent22              

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Free keywords: Amino Acid Sequence; Bacterial Proteins/ch [Chemistry]; *Bacterial Proteins/ul [Ultrastructure]; Chaperonins; Chromatography, Gel; *Cysteine Endopeptidases/ul [Ultrastructure]; Electrophoresis, Polyacrylamide Gel; *Escherichia coli/ul [Ultrastructure]; *Eubacterium/ul [Ultrastructure]; GroEL Protein; Heat-Shock Proteins/ch [Chemistry]; *Heat-Shock Proteins/ul [Ultrastructure]; Microscopy, Electron; Molecular Sequence Data; *Multienzyme Complexes/ul [Ultrastructure]; *Proteins/ch [Chemistry]; Sequence Homology, Nucleic Acid
 Abstract: In an attempt to settle the question of whether the multicatalytic proteinase or proteasome exist in all three kingdoms of life--eukaryotes, archaebacteria, and eubacteria--we have undertaken a search for them in the eubacterium Comamonas acidovorans. We have, in fact, isolated and purified a cylinder-shaped particle. However, according to various structural and biochemical criteria this turned out to be more reminiscent of the groEL protein from Escherichia coli and its homologs than to proteasomes of eukaryotic or archaebacterial origin. N-terminal sequencing provided definite proof for its belonging to this family of molecular chaperonins. Image analysis of electron micrographs revealed that the C. acidovorans groEL-like protein and proteasomes in spite of their significantly different dimensions have certain principles of organization in common.

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 Dates: 1990
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: eDoc: 318425
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Title: Journal of Structural Biology.
Source Genre: Journal
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Pages: - Volume / Issue: 103 (3) Sequence Number: - Start / End Page: 197 - 203 Identifier: -