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  Proteasomes from Dictyostelium discoideum: characterization of structure and function

Schauer, T. M., Nesper, M., Kehl, M., Lottspeich, F., Müller-Taubenberger, A., Gerisch, G., et al. (1993). Proteasomes from Dictyostelium discoideum: characterization of structure and function. Journal of Structural Biology., 111(2), 135-147.

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Schauer, T. M., Autor
Nesper, M., Autor
Kehl, M., Autor
Lottspeich, F.1, Autor           
Müller-Taubenberger, A.1, Autor           
Gerisch, G.1, Autor           
Baumeister, W.1, Autor           
Affiliations:
1External Organizations, ou_persistent22              

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Schlagwörter: Amino Acid Sequence; Animal; Cloning, Molecular; *Cysteine Endopeptidases/ch [Chemistry]; Cysteine Endopeptidases/ge [Genetics]; Cysteine Endopeptidases/me [Metabolism]; DNA, Complementary/ge [Genetics]; *Dictyostelium/en [Enzymology]; Dictyostelium/ge [Genetics]; Dictyostelium/gd [Growth & Development]; Electrophoresis, Gel, Two-Dimensional; Immunoblotting; Microscopy, Electron; Molecular Sequence Data; Molecular Structure; *Multienzyme Complexes/ch [Chemistry]; Multienzyme Complexes/ge [Genetics]; Multienzyme Complexes/me [Metabolism]; Oligopeptides/ch [Chemistry]; Protein Conformation; Substrate Specificity
 Zusammenfassung: We have isolated and purified 20 S proteasomes from Dictyostelium discoideum and characterized their proteolytic activities. Two-dimensional electrophoresis revealed 13 distinct spots. Affinity purification with a subunit-specific monoclonal antibody indicated that the preparation was homogeneous, i.e., each individual proteasome appeared to have the full set of subunits. We have not obtained any evidence for changes in subunit composition at different developmental stages. The cDNA clones coding for two subunits (4 and 5), both of the alpha-type, have been cloned and sequenced. It has been shown by immunoelectron microscopy that each proteasome is composed of two identical halves, related to each other by C2 symmetry. The resulting model implies that the alpha- and beta-subunits have a fixed pattern of relationships. D. discoideum proteasomes are found both in the cytosol and, in higher concentrations, in the nucleus.

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 Datum: 1993
 Publikationsstatus: Erschienen
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 Identifikatoren: eDoc: 318358
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Titel: Journal of Structural Biology.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 111 (2) Artikelnummer: - Start- / Endseite: 135 - 147 Identifikator: -