hide
Free keywords:
Escherichia-coli; Biological particles; Ribosome; Reconstruction; Micrographs; Microscopy; Resolution; Images.; Biochemistry & biophysics.
Abstract:
From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. (C) 1997 Academic Press. [References: 24]
