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  Late events in the assembly of 20S proteasomes

Mayr, U., Seemüller, E., Müller, S. A., Engel, A., & Baumeister, W. (1998). Late events in the assembly of 20S proteasomes. Journal of Structural Biology, 124(2-3), 179-188.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7248-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7249-9
Genre: Journal Article
Alternative Title : J. Struct. Biol

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Mayr, U., Author
Seemüller, E.1, Author              
Müller, S. A., Author
Engel, A., Author
Baumeister, W.1, Author              
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1External Organizations, escidoc:persistent22              

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Free keywords: Thermoplasma-acidophilum; Precursor complexes; Electron-microscopy; Antigenic peptides; Beta-subunits; Proteins; Degradation; Rhodococcus; Maturation; Cleavage.; Biochemistry & Biophysics in Current Contents(R)/Life Sciences.
 Abstract: Electron microscopy and STEM mass measurements have been used to characterize late intermediates in the assembly pathway of wildtype and mutant Rhodococcus proteasomes. A proteolytically inactive and processing-incompetent mutant, beta K33A, allowed a short-lived late intermediate of the pathway to be captured, the preholoproteasome. In this fully assembled 20S complex the 14 propeptides with an aggregate mass of 100 kDa fill the whole central cavity and most of the two antechambers. It is further shown that in wildtype Rhodococcus proteasomes the propeptides are degraded in a processive manner undergoing multiple cleavages before the products are discharged and the inner cavities are cleared. It appears that the docking of two half-proteasomes, i.e., preholoproteasome formation, is sufficient to trigger autocleavage of the Gly-1/Thr1 bond necessary for active site formation and the subsequent degradation of the propeptides. (C) 1998 Academic Press. [References: 33]

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 Dates: 1998
 Publication Status: Published in print
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 Identifiers: eDoc: 318764
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Title: Journal of Structural Biology
  Alternative Title : J. Struct. Biol
Source Genre: Journal
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Pages: - Volume / Issue: 124 (2-3) Sequence Number: - Start / End Page: 179 - 188 Identifier: -