English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway

Mello, J. A., Sillje, H. H. W., Roche, D. M. J., Kirschner, D. B., Nigg, E. A., & Almouzni, G. (2002). Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway. EMBO Reports, 3(4), 329-334.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : EMBO Rep.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Mello, J. A., Author
Sillje, H. H. W.1, Author
Roche, D. M. J., Author
Kirschner, D. B., Author
Nigg, E. A.2, Author           
Almouzni, G., Author
Affiliations:
1External Organizations, ou_persistent22              
2Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

Content

show
hide
Free keywords: -
 Abstract: The efficient assembly of newly replicated and repaired DNA into chromatin is essential for proper genome function. Based on genetic studies in Saccharomyces cerevisiae, the histone chaperone anti-silencing function 1 (Asf1) has been implicated in the DNA repair response. Here, the human homologs are shown to function synergistically with human CAF-1 to assemble nucleosomes during nucleotide excision repair in vitro. Furthermore, we demonstrate that hAsf1 proteins can interact directly with the p60 subunit of hCAF-1. In contrast to hCAF-1 p60, the nuclear hAsf1 proteins are not significantly associated with chromatin in cells before or after the induction of DNA damage, nor specifically recruited to damaged DNA during repair in a bead-linked DNA assay. A model is proposed in which the synergism between hAsf1 and CAF-1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF-1.

Details

show
hide
Language(s): eng - English
 Dates: 2002-04
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 39138
ISI: 000175273700012
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: EMBO Reports
  Alternative Title : EMBO Rep.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 (4) Sequence Number: - Start / End Page: 329 - 334 Identifier: ISSN: 1469-221X