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  A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acids

Kokoeva, M. V., Storch, K. F., Klein, C., & Oesterhelt, D. (2002). A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acids. EMBO Journal, 21(10), 2312-2322.

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Genre: Zeitschriftenartikel
Alternativer Titel : Embo J.

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 Urheber:
Kokoeva, M. V.1, Autor           
Storch, K. F.1, Autor           
Klein, C.1, Autor           
Oesterhelt, D.1, Autor           
Affiliations:
1Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Schlagwörter: compatible solute; glycine betaine; lipoprotein; methyl- accepting chemotaxis; osmotic stress
 Zusammenfassung: Directly upstream of the Halobacterium salinarum transducer genes basT and htpIV we identified two open reading frames (orfs) with significant homologies to genes encoding binding proteins for amino acids and compatible solutes, respectively. Behavioral testing of deletion mutants indicates that halobacterial chemotaxis towards branched-chain amino acids as well as compatible osmolytes of the betaine family requires both a binding and a transducer protein. We therefore named the binding/transducer proteins BasB/BasT for branched-chain and sulfur-containing amino acids and CosB/CosT for compatible solutes. Our data support a signaling mechanism with the binding proteins functioning as lipid-anchored receptors interacting with the extracellular domain of their cognate transducers. Inspection of the halobacterial genome suggests that BasB and CosB exclusively mediate chemotaxis responses without any additional role in transport, which is in contrast to bacterial binding proteins, which are always part of ABC transport systems. The CosB/CosT system is the first instance of a chemotaxis signaling pathway for organic osmolytes in the living world and natural abundance C-13-NMR analysis of cytoplasmic extracts suggests that H.salinarum utilizes these solutes for osmotic adaptation.

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Sprache(n): eng - English
 Datum: 2002-05-15
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 38369
ISI: 000175753600002
 Art des Abschluß: -

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Titel: EMBO Journal
  Alternativer Titel : Embo J.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 21 (10) Artikelnummer: - Start- / Endseite: 2312 - 2322 Identifikator: ISSN: 0261-4189