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  Human TPX2 is required for targeting Aurora-A kinase to the spindle

Kufer, T. A., Sillje, H. H. W., Körner, R., Gruss, O. J., Meraldi, P., & Nigg, E. A. (2002). Human TPX2 is required for targeting Aurora-A kinase to the spindle. Journal of Cell Biology, 158(4), 617-623.

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Genre: Journal Article
Alternative Title : J. Cell Biol.

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 Creators:
Kufer, T. A.1, Author           
Sillje, H. H. W.2, Author
Körner, R.1, 3, Author           
Gruss, O. J., Author
Meraldi, P.1, Author           
Nigg, E. A.1, Author           
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              
2External Organizations, ou_persistent22              
3Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              

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Free keywords: spindle; mitosis; Aurora-A; TPX2; siRNA
 Abstract: Aurora-A is a serine-threonine kinase implicated in the assembly and maintenance of the mitotic spindle. Here we show that human Aurora-A binds to TPX2, a prominent component of the spindle apparatus. TPX2 was identified by mass spectrometry as a major protein coimmunoprecipitating specifically with Aurora- A from mitotic HeLa cell extracts. Conversely, Aurora-A could be detected in TPX2 immunoprecipitates. This indicates that subpopulations of these two proteins undergo complex formation in vivo. Binding studies demonstrated that the NH2 terminus of TPX2 can directly interact with the COOH-terminal catalytic domain of Aurora-A. Although kinase activity was not required for this interaction, TPX2 was readily phosphorylated by Aurora-A. Upon siRNA-mediated elimination of TPX2 from cells, the association of Aurora-A with the spindle microtubules was abolished, although its association with spindle poles was unaffected. Conversely, depletion of Aurora-A by siRNA had no detectable influence on the localization of TPX2. We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly.

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Language(s): eng - English
 Dates: 2002-08-19
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41118
ISI: 000177649900003
 Degree: -

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Title: Journal of Cell Biology
  Alternative Title : J. Cell Biol.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 158 (4) Sequence Number: - Start / End Page: 617 - 623 Identifier: ISSN: 0021-9525