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  RNA DNA discrimination by the antitermination protein NusB

Mühlberger, R., Robelek, R., Eisenreich, W., Ettenhuber, C., Sinner, E. K., Kessler, H., et al. (2003). RNA DNA discrimination by the antitermination protein NusB. Journal of Molecular Biology, 327(5), 973-983.

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Genre: Zeitschriftenartikel
Alternativer Titel : J. Mol. Biol.

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 Urheber:
Mühlberger, R., Autor
Robelek, R., Autor
Eisenreich, W., Autor
Ettenhuber, C., Autor
Sinner, E. K.1, Autor           
Kessler, H., Autor
Bacher, A., Autor
Richter, G., Autor
Affiliations:
1Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Schlagwörter: antitermination; NMR spectroscopy; surface plasmon resonance spectroscopy; protein RNA interaction; DNA RNA discrimination
 Zusammenfassung: The regulation of ribosomal RNA biosynthesis in Escherichia coli by anti-termination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA. The affinity of NusB protein for boxA RNA exceeds that for the homologous DNA segment by more than three orders of magnitude as shown by surface plasmon resonance measurements. DNA RNA discrimination by NusB protein was shown to involve methyl groups (i.e. discrimination of uracil versus thymine) and 2' hydroxyl groups (i.e. discrimination of ribose versus deoxyribose side-chains) in the RNA motif. Ligand perturbation experiments monitored by (HN)-H-1-N-15 correlation NMR experiments identified amide NH groups whose chemical shifts are affected selectively by ribose/deoxyribose exchange in the 5' and the central part of the dodecameric boxA motif respectively. The impact of structural modification of the boxA motif on the affinity for NusB protein as observed by (HN)-H-1- N-15 heterocorrelation was analysed by a generic algorithm. (C) 2003 Elsevier Science Ltd. All rights reserved.

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Sprache(n): eng - English
 Datum: 2003-04-11
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 41412
ISI: 000182093300007
 Art des Abschluß: -

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Titel: Journal of Molecular Biology
  Alternativer Titel : J. Mol. Biol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 327 (5) Artikelnummer: - Start- / Endseite: 973 - 983 Identifikator: ISSN: 0022-2836