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  Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model

Direnberger, S., Mues, M., Micale, V., Wotjak, C. T., Dietzel, S., Schubert, M., et al. (2012). Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model. NATURE COMMUNICATIONS, 3: 1031. doi:10.1038/ncomms2035.

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 Creators:
Direnberger, Stephan1, Author           
Mues, Marsilius2, Author           
Micale, Vincenzo3, Author
Wotjak, Carsten T.3, Author
Dietzel, Steffen3, Author
Schubert, Michael3, Author
Scharr, Andreas3, Author
Hassan, Sami3, Author
Wahl-Schott, Christian3, Author
Biel, Martin3, Author
Krishnamoorthy, Gurumoorthy2, Author           
Griesbeck, Oliver1, Author           
Affiliations:
1Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              
2Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              
3external, ou_persistent22              

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Free keywords: GREEN FLUORESCENT PROTEIN; TROPONIN-C; IN-VIVO; SINOATRIAL NODE; RESISTANCE ARTERIES; CA2+ INDICATORS; RHOA/RHO-KINASE; MICE; EXPRESSION; ACTIVATION
 Abstract: Engineering efforts of genetically encoded calcium indicators predominantly focused on enhancing fluorescence changes, but how indicator expression affects the physiology of host organisms is often overlooked. Here, we demonstrate biocompatibility and widespread functional expression of the genetically encoded calcium indicator TN-XXL in a transgenic mouse model. To validate the model and characterize potential effects of indicator expression we assessed both indicator function and a variety of host parameters, such as anatomy, physiology, behaviour and gene expression profiles in these mice. We also demonstrate the usefulness of primary cells and organ explants prepared from these mice for imaging applications. Although we find mild signatures of indicator expression that may be further reduced in future sensor generations, the 'green' indicator mice generated provide a well-characterized resource of primary cells and tissues for in vitro and in vivo calcium imaging applications.

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Language(s): eng - English
 Dates: 2012-08-28
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000308801100046
DOI: 10.1038/ncomms2035
 Degree: -

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Title: NATURE COMMUNICATIONS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 3 Sequence Number: 1031 Start / End Page: - Identifier: ISSN: 2041-1723