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  Lack of endothelial diaphragms in fenestrae and caveolae of mutant Plvap-deficient mice

Herrnberger, L., Seitz, R., Kuespert, S., Boesl, M. R., Fuchshofer, R., & Tamm, E. R. (2012). Lack of endothelial diaphragms in fenestrae and caveolae of mutant Plvap-deficient mice. HISTOCHEMISTRY AND CELL BIOLOGY, 138(5), 709-724. doi:10.1007/s00418-012-0987-3.

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 Creators:
Herrnberger, Leonie1, Author
Seitz, Roswitha1, Author
Kuespert, Sabrina1, Author
Boesl, Michael R.2, Author           
Fuchshofer, Rudolf1, Author
Tamm, Ernst R.1, Author
Affiliations:
1external, ou_persistent22              
2Department: Molecular Neurobiology / Klein, MPI of Neurobiology, Max Planck Society, ou_1113546              

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Free keywords: RECEPTOR TYROSINE KINASE; CAPILLARY ENDOTHELIUM; DIFFERENTIATED MICRODOMAINS; LUMINAL SURFACE; PHENOTYPIC HETEROGENEITY; ANIONIC SITES; KNOCKOUT MICE; DEFECTS; MOUSE; PV-1Vascular endothelium; Capillaries; Kidney; Pancreas; Cardiac defects;
 Abstract: Plasmalemmal vesicle-associated protein (PLVAP, PV-1) is specifically expressed in endothelial cells in which it localizes to diaphragms of fenestrae, caveolae, and transendothelial channels. To learn about its function, we generated mutant mice that lack PLVAP. In a C57BL/6N genetic background, homozygous Plvap-deficient embryos die before birth and suffer from subcutaneous edema, hemorrhages, and defects in the vascular wall of subcutaneous capillaries. In addition, hearts of Plvap (-/-) embryos show ventricular septal defects and thinner ventricular walls. In wild-type embryos, PLVAP and caveolae with a stomatal diaphragm are present in endothelial cells of subcutaneous capillaries and endocardium, while a diaphragm is missing in caveolae of Plvap (-/-) littermates. Plvap (-/-) mice in a mixed C57BL/6N/FVB-N genetic background are born and survive at the most for 4 weeks. Capillaries of exocrine and endocrine pancreas and of kidney peritubular interstitium were investigated in more detail as examples of fenestrated capillaries. In these vascular beds, Plvap (-/-) mice show a complete absence of diaphragms in fenestrae, caveolae, and transendothelial channels, findings which are associated with a substantial decrease in the number of endothelial fenestrae. The changes in the capillary phenotype correlate with a considerable retardation of postnatal growth and anemia. Plvap (-/-) mice provide an animal model to clarify the specific functional role of endothelial fenestrae and their contribution to passage of water and solutes in different organs.

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Language(s): eng - English
 Dates: 2012-11
 Publication Status: Issued
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000309873300001
DOI: 10.1007/s00418-012-0987-3
 Degree: -

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Title: HISTOCHEMISTRY AND CELL BIOLOGY
Source Genre: Journal
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Publ. Info: 233 SPRING ST, NEW YORK, NY 10013 USA : SPRINGER
Pages: - Volume / Issue: 138 (5) Sequence Number: - Start / End Page: 709 - 724 Identifier: ISSN: 0948-6143