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  Amplification of hypervariable simple sequence repeats (microsatellites) from excremental DNA of wild living bonobos (Pan paniscus)

Gerloff, U., Schlötterer, C., Rassmann, K., Rambold, I., Hohmann, G., Fruth, B., et al. (1995). Amplification of hypervariable simple sequence repeats (microsatellites) from excremental DNA of wild living bonobos (Pan paniscus). Molecular Ecology, 4(4), 515-518. doi:10.1111/j.1365-294X.1995.tb00247.x.

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Gerloff, U.1, Autor
Schlötterer, C.1, Autor
Rassmann, K.1, Autor
Rambold, I.1, Autor
Hohmann, G.1, Autor
Fruth, B.1, Autor
Tautz, D.2, Autor           
Affiliations:
1external, ou_persistent22              
2External, ou_persistent22              

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 Zusammenfassung: We show that nuclear DNA extracted from faeces of free living bonobos (Pan paniscus) can be used to amplify hypervariable simple sequence repeats, which can be used for paternity analysis and kinship studies. Of 130 DNA extractions of samples from 33 different animals, about two-thirds yielded PCR products at the first attempt. For several samples only a second extraction resulted in positive amplifications. Consistency tests revealed that in some cases only one of the two alleles was amplified. Presumably this is due to a very limited amount of bonobo DNA in the sample and we suggest therefore that a sample found to be homozygous at a given locus should be typed repeatedly for verification.

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 Datum: 1995-081995-08
 Publikationsstatus: Erschienen
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Titel: Molecular Ecology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Oxford : Blackwell Science
Seiten: - Band / Heft: 4 (4) Artikelnummer: - Start- / Endseite: 515 - 518 Identifikator: ISSN: 0962-1083
CoNE: https://pure.mpg.de/cone/journals/resource/954925580119