English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples.

Braker, G., Fesefeldt, A., & Witzel, K.-P. (1998). Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples. Applied and Environmental Microbiology, 64(10), 3769-3775.

Item is

Files

show Files
hide Files
:
Braker, G., et al., 1998, S-37363.pdf (Publisher version), 171KB
 
File Permalink:
-
Name:
Braker, G., et al., 1998, S-37363.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, MPLM; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Braker, Gesche1, Author           
Fesefeldt, Andreas, Author
Witzel, Karl-Paul1, Author           
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              

Content

show
hide
Free keywords: -
 Abstract: A system was developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with PCR, Primer sequences were found for the amplification of fragments from both nitrite reductase genes (nirK and nirS) after comparative sequence analysis. Whenever amplification was tried with these primers, the known nir type of denitrifying laboratory cultures could be confirmed. Likewise, the method allowed a determination of the nir type of five laboratory strains. The nirK gene could be amplified from Blastobacter denitrificans, Alcaligenes xylosoxidans, and Alcaligenes sp, (DSM 30128); the nirS gene was amplified from Alcaligenes eutrophus DSM 530 and from the denitrifying isolate IFAM 3698, For each of the two genes, at least one primer combination amplified successfully for all of the test strains. Specific amplification products were not obtained,vith nondenitrifying bacteria or with strains of the other nir type. The specificity of the amplified products was confirmed by subsequent sequencing. These results suggest the suitability of the method for the qualitative detection of denitrifying bacteria in environmental samples. This was shown by applying one generally amplifying primer combination for each nir gene developed in this study to total DNA preparations from aquatic habitats.

Details

show
hide
Language(s): eng - English
 Dates: 1998-10
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 120977
Other: 1716/S 37363
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied and Environmental Microbiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 64 (10) Sequence Number: - Start / End Page: 3769 - 3775 Identifier: ISSN: 0099-2240