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  Identification and characterization of 10 microsatellite primers for the calanoid freshwater copepods Eudiaptomus gracilis and E-graciloides using enriched genomic libraries

Zeller, M., & Reusch, T. B. H. (2004). Identification and characterization of 10 microsatellite primers for the calanoid freshwater copepods Eudiaptomus gracilis and E-graciloides using enriched genomic libraries. Molecular Ecology Notes, 4(3), 355-357. doi:10.1111/j.1471-8286.2004.00695.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DA87-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DA88-D
Genre: Journal Article

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zeller_2004.pdf (Publisher version), 83KB
 
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 Creators:
Zeller, Martina1, Author              
Reusch, Thorsten B. H.1, 2, Author              
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, escidoc:976547              
2Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, escidoc:1445634              

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Free keywords: copepods; cross-amplification; Eudiaptomus gracilis; Eudiaptomus graciloides; genomic library; microsatellites
 Abstract: The calanoid copepods Eudiaptomus gracilis and Eudiaptomus graciloides are widespread among European lakes. We constructed enriched genomic libraries for both species in order to isolate and characterise microsatellite markers. The obtained 7 polymorphic microsatellite-loci for E. gracilis and 3 for E. graciloides are the first for any freshwater copepod. They display an expected heterozygosity ranging from 0.60 to 0.96 and 0.63 to 0.94 and observed heterozygosity ranging from 0.53 to 0.87 and 0.57 to 0.87, respectively. We were not able to cross-amplify the isolated loci across species, indicating long divergence among both congeneric species despite morphological similarity

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Language(s): eng - English
 Dates: 2004-09
 Publication Status: Published in print
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 Identifiers: eDoc: 200405
DOI: 10.1111/j.1471-8286.2004.00695.x
Other: 2311/S 38186
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Title: Molecular Ecology Notes
Source Genre: Journal
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Pages: - Volume / Issue: 4 (3) Sequence Number: - Start / End Page: 355 - 357 Identifier: ISSN: 1471-8278