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  Isolation of polymorphic microsatellite loci in the clear lake gnat and two other phantom midge species of the genus Chaoborus (Diptera : Chaoboridae)

Berendonk, T. U., & Dobson, K. (2006). Isolation of polymorphic microsatellite loci in the clear lake gnat and two other phantom midge species of the genus Chaoborus (Diptera: Chaoboridae). Molecular Ecology Notes, 6(1), 17-19. doi:10.1111/j.1471-8286.2005.01102.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D8D1-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D8D2-6
Genre: Journal Article

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berendonk_2006.pdf (Publisher version), 67KB
 
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 Creators:
Berendonk, T. U.1, Author              
Dobson, K., Author
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, escidoc:976547              

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Free keywords: Chaoborus; C. americanus; C. astictopus; C. punctipennis; freshwater; microsatellites
 Abstract: Chaoborus is of great interest to many freshwater ecologists. The adults can become pests in certain areas in North America and the larvae are an important food source for fish. In this preliminary study, we identified variable microsatellite loci in three species: Chaoborus astictopus (H-E = 0.52-0.76), Chaoborus americanus (H-E = 0.46-0.80) and Chaoborus punctipennis (H-E = 0.66-0.81). Using a biotin/streptavidin capture technique of repetitive sequences in a 96-well format, we obtained microsatellite-enriched genomic libraries for all three species and identified six polymorphic microsatellite markers for each species. None of the primers did yield a polymerase chain reaction fragment in a cross-species test.

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Language(s): eng - English
 Dates: 2006-03
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: eDoc: 261791
DOI: 10.1111/j.1471-8286.2005.01102.x
Other: 2454/S 38469
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Title: Molecular Ecology Notes
  Alternative Title : Mol. Ecol. Notes
Source Genre: Journal
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Pages: - Volume / Issue: 6 (1) Sequence Number: - Start / End Page: 17 - 19 Identifier: ISSN: 1471-8278