English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 Local TagsRelease HistoryDetailsSummary
  Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco, a high altitude saline wetland in northern Chile

Dorador, C., Busekow, A., Vila, I., Imhoff, J. F., & Witzel, K.-P. (2008). Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco, a high altitude saline wetland in northern Chile. Extremophiles, 12(3), 405-414. doi:10.1007/s00792-008-0146-x.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-D6B1-2 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-D6B2-F
Genre: Journal Article

Files

show Files
hide Files
:
Dorador, C., et al., 2008, S-38761.pdf (Publisher version), 285KB
 
File Permalink:
-
Name:
Dorador, C., et al., 2008, S-38761.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, MPLM; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Dorador, Cristina1, Author           
Busekow, Annika1, Author           
Vila, Irma, Author
Imhoff, Johannes F., Author
Witzel, Karl-Paul1, Author           
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              

Content

show
hide
Free keywords: ammonia-oxidizing bacteria; 16S rDNA; amoA gene; andean altiplano; salinity
 Abstract: We analyzed enrichment cultures of ammonia-oxidizing bacteria (AOB) collected from different areas of Salar de Huasco, a high altitude, saline, pH-neutral water body in the Chilean Altiplano. Samples were inoculated into mineral media with 10 mM NH4+ at five different salt concentrations (10, 200, 400, 800 and 1,400 mM NaCl). Low diversity (up to three phylotypes per enrichment) of beta-AOB was detected using 16S rDNA and amoA clone libraries. Growth of beta-AOB was only recorded in a few enrichment cultures and varied according to site or media salinity. In total, five 16S rDNA and amoA phylotypes were found which were related to Nitrosomonas europaea/Nitrosococcus mobilis, N. marina and N. communis clusters. Phylotype 1-16S was 97% similar with N. halophila, previously isolated from Mongolian soda lakes, and phylotypes from amoA sequences were similar with yet uncultured beta-AOB from different biofilms. Sequences related to N. halophila were frequently found at all salinities. Neither gamma-AOB nor ammonia-oxidizing Archaea were recorded in these enrichment cultures.

Details

show
hide
Language(s): eng - English
 Dates: 2008-05
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 361812
DOI: 10.1007/s00792-008-0146-x
Other: 2598/S 38761
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Extremophiles
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 12 (3) Sequence Number: - Start / End Page: 405 - 414 Identifier: ISSN: 1431-0651