English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Copy number variants and their role in adaptations in wild populations of Mus musculus domesticus

Nitsche, J. (2010). Copy number variants and their role in adaptations in wild populations of Mus musculus domesticus. Diploma Thesis, Fachhochschule, Bingen.

Item is

Files

show Files
hide Files
:
Diplomarbeit_19.02.10.pdf (Publisher version), 704KB
 
File Permalink:
-
Name:
Diplomarbeit_19.02.10.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, MPLM; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Nitsche, Jessica1, Author           
Krefft, Marianne, Advisor
Bryk, Jarek1, Advisor           
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

Content

show

Details

show
hide
Language(s): eng - English
 Dates: 2010-01-31
 Publication Status: Accepted / In Press
 Pages: 62 Bl.
 Publishing info: Bingen : Fachhochschule
 Table of Contents: Table of contents
List of Abbreviations...................................................................................................5
List of figures..............................................................................................................6
List of tables...............................................................................................................7
Acknowledgments......................................................................................................8
1. Introduction.............................................................................................................9
1.1 Natural selection and adaptations.....................................................................9
1.2 The worldwide migration of Mus musculus domesticus..................................12
1.3 Copy number variants.....................................................................................14
1.4 Molecular signature of recent positive selection..............................................16
1.5 Microsatellites as markers of genetic variability..............................................17
1.6 Goal and overview of the study.......................................................................19
2. Materials and methods.........................................................................................20
2.1 Materials.........................................................................................................20
2.1.1 Lab equipment..........................................................................................20
2.1.2 Chemicals.................................................................................................21
2.1.3 Programmes.............................................................................................22
2.1.4 Animals.....................................................................................................23
2.2 Methods..........................................................................................................24
2.2.1 DNA isolation and purification..................................................................24
2.2.2 Polymerase chain reaction (PCR)............................................................24
2.2.3 Agarose gel electrophoresis.....................................................................25
2.2.4 Primer design for qPCR............................................................................26
2.2.5 Microsatellite PCR and sequencing..........................................................26
3. Results..................................................................................................................28
3.1 Design of the primers to amplify putative copy-number variable regions........28
3.1 Primer efficiency test.......................................................................................30
3.2 Quantitative PCR............................................................................................34
3.4 Microsatellite scan for selective sweeps.........................................................38
4. Discussion...........................................................................................................41
4.1. Technical issues concerning quantitative PCR assays..................................41
4.2. Results of the quantitative PCR assays.........................................................42
4.3. Results of the microsatellite screen................................................................43
4.4. Future work........................................................................................................44
6. References...........................................................................................................45
7. Attachment...........................................................................................................52
 Rev. Type: -
 Identifiers: eDoc: 453067
Other: Diss/12200
 Degree: Diploma

Event

show

Legal Case

show

Project information

show

Source

show