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Wolbachia; Apis mellifera carnica; 16S ribosomal RNA gene; PCR
Abstract:
Intracellular endosymbiont, Wolbachia spp., have been previously reported in many different orders
of insects, but not in the European honeybee sub species Apis mellifera carnica. We first report the presence
of Wolbachia spp. in the honeybee sub spp. Apis mellifera carnica of the order Hymenoptera. Using standard
polymerase chain reaction (PCR) targeting of the 16S ribosomal RNA gene, we screened for Wolbachia spp.
in honeybee Queen and workers collected from different parts of Germany. Results of the study revealed 100%
infection status of Wolbachia in Apis mellifera carnica collected from different parts of Germany, confirming
that the Wolbachia spp. were invariably present in the honeybee Queen and the workers screened.
