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  An autoimmunity odyssey: how autoreactive T cells infiltrate into the CNS

Kawakami, N., Bartholomäus, I., Pesic, M., & Mues, M. (2012). An autoimmunity odyssey: how autoreactive T cells infiltrate into the CNS. IMMUNOLOGICAL REVIEWS, 248(Sp. Iss.), 140-155. doi:10.1111/j.1600-065X.2012.01133.x.

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 Creators:
Kawakami, Naoto1, Author           
Bartholomäus, Ingo1, Author           
Pesic, Marija1, Author           
Mues, Marsilius1, Author           
Affiliations:
1Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              

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Free keywords: CENTRAL-NERVOUS-SYSTEM; MULTIPLE-SCLEROSIS; IN-VIVO; MOLECULAR-MECHANISMS; ANTIGEN PRESENTATION; 2-PHOTON MICROSCOPY; P-SELECTIN; ENCEPHALOMYELITIS; FLUORESCENCE; PROTEINexperimental autoimmune encephalomyelitis; autoimmunity; T cells; two-photon intravital microscopy;
 Abstract: Experimental autoimmune encephalomyelitis (EAE) is a widely used animal model of multiple sclerosis (MS), a human autoimmune disease. To explore how EAE and ultimately MS is induced, autoantigen-specific T cells were established, were labeled with fluorescent protein by retroviral gene transfer, and were tracked in vivo after adoptive transfer. Intravital imaging with two-photon microscopy was used to record the entire entry process of autoreactive T cells into the CNS: a small number of T cells first appear in the CNS leptomeninges before onset of EAE, and crawl on the intraluminal surface of blood vessels, which is integrin a4 and aL dependent. After extravasation, the T cells continue into the perivascular space, meeting local antigen-presenting cells (APCs), which present endogenous antigens. This interaction activates the T cells and guides them to penetrate the CNS parenchyma. As the local APCs in the CNS are not saturated with endogenous antigens, exogenous antigens stimulate the autoreactive T cells more strongly and, as a result, exacerbate the clinical outcome. Currently, we are attempting to visualize T-cell activation in vivo in both rat T-cell-mediated EAE and mouse spontaneous EAE models.

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Language(s): eng - English
 Dates: 2012-07
 Publication Status: Issued
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: IMMUNOLOGICAL REVIEWS
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 248 (Sp. Iss.) Sequence Number: - Start / End Page: 140 - 155 Identifier: ISSN: 0105-2896