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  Biotin-Streptavidin Sensor Surface: a Versatile Platform for Performing DNA Hybridization Interactions

Kambhampati, D., & Knoll, W. (2002). Biotin-Streptavidin Sensor Surface: a Versatile Platform for Performing DNA Hybridization Interactions. In D. Möbius, & R. Miller (Eds.), Organized Monolayers and Assemblies: Structure, Processes and Function (pp. 279-316). Elsevier.

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 Creators:
Kambhampati, D.1, Author           
Knoll, Wolfgang1, Author           
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

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 Abstract: The properties of the biotin-streptavidin sensor system such as surface topology, regeneration of probes and non-specific interaction effects are investigated in detail by using DNA-DNA hybridization interactions as the test system. Surface Plasmon Resonance (SPR) and SPR enhanced fluorescence spectroscopy (SPRFS) detection schemes were widely employed to monitor the effect of probe/target length, mismatches and temperature on the hybridization kinetics. 1:1 Langmuir kinetic models were found to be sufficient to describe the hybridization interactions.

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Language(s): eng - English
 Dates: 2002
 Publication Status: Issued
 Pages: -
 Publishing info: Elsevier
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 42681
Other: P-03-13
 Degree: -

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Title: Organized Monolayers and Assemblies: Structure, Processes and Function
Source Genre: Book
 Creator(s):
Möbius, Dietmar, Editor
Miller, Reinhard, Editor
Affiliations:
-
Publ. Info: Elsevier
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 279 - 316 Identifier: -

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Title: Studies in Interface Science
Source Genre: Series
 Creator(s):
Möbius, D., Editor
Miller, R., Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: 16 Sequence Number: - Start / End Page: - Identifier: -