Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Photochemically induced dynamic nuclear polarisation in entire bacterial photosynthetic units observed by 13C magic-angle spinning NMR

Prakash, S., Alia, A., Jeschke, G., de Groot, H. J. M., & Matysik, J. (2003). Photochemically induced dynamic nuclear polarisation in entire bacterial photosynthetic units observed by 13C magic-angle spinning NMR. Journal of Molecular Structure, 661-662, 625-633.

Item is

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Prakash, S., Autor
Alia, A., Autor
Jeschke, Gunnar1, Autor           
de Groot, H. J. M., Autor
Matysik, J., Autor
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Photochemically induced dynamic nuclear polarisation has been observed from entire photosynthetic units (PSUs) bound to chromatophore membrane (membrane-bound PSU) of the purple bacteria Rhodobacter sphaeroides, which have been selectively 13C-isotope enriched at all BChl and BPheo cofactors. These 1.5 MDa membrane-bound protein complexes comprise reaction centres as well as the antenna systems called light harvesting complexes I and II. Due to light-induced enhancement of nuclear polarisation, the 13C magic-angle spinning (MAS) NMR spectrum shows absorptive lines originating from the cofactors involved into the photochemical machinery and allowing the determination of the electronic ground state structure at atomic resolution. Addition of detergent released intact PSU from the chromatophore membrane (so called detergent solubilised PSU) and caused significant changes in the sign and intensity pattern of the light-induced MAS NMR spectrum. In contrast, detergent solubilised PSU and detergent solubilised bacterial reaction centres with the same isotope label pattern exhibit essentially the same chemical shifts with only minor differences in the intensity pattern. The pronounced differences between intact membrane-bound and detergent solubilised PSU are tentatively explained by the loss of self-orientation of the membrane-bound samples by solubilisation. This interpretation suggests that the theoretically predicted anisotropy of the light-induced nuclear polarisation has been observed for the first time.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2003
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 60315
Anderer: P-03-91
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of Molecular Structure
  Alternativer Titel : J. Mol. Struct.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 661-662 Artikelnummer: - Start- / Endseite: 625 - 633 Identifikator: -