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  Supramolecular functional interfacial architectures for biosensor applications

Knoll, W., Yu, F., Neumann, T., Schiller, S., & Naumann, R. (2003). Supramolecular functional interfacial architectures for biosensor applications. Physical Chemistry Chemical Physics, 5(23), 5169-5175.

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 Creators:
Knoll, Wolfgang1, Author           
Yu, F.1, Author           
Neumann, T.1, Author           
Schiller, Stefan1, Author           
Naumann, Renate1, Author           
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

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 Abstract: This short review describes some of our efforts in generating bio-functional supramolecular interfacial architectures for their use as affinity coatings in biosensor development based on a recently introduced novel optical recording principle combining the resonant excitation of surface plasmon modes and fluorescence detection schemes. Examples are given for multilayer assemblies designed for surface hybridization reactions between a grafted oligonucleotide catcher probe and target strands from solution. By describing the binding behavior of fluorophore-labeled rabbit–anti-mouse IgG to surface-attached mouse IgG, the limit of detection of the surface plasmon fluorescence spectroscopy will be shown to be in the lower femtomolar concentration range. These DNA-and protein binding studies will be complemented by examples for membrane-based biosensor platforms. We will document that tethered lipid bilayer membranes can be assembled with specific capacities of Cm=0.5µF cm–2 and specific resistivities in excess of Rm>20 MΩ cm2. The incorporation of the synthetic ionophore valinomycin can mediate the K+-ion translocation across these tethered membranes, thus reducing the resistivity selectively and reversibly by more than four orders of magnitude.

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Language(s): eng - English
 Dates: 2003
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 59997
Other: P-03-87
 Degree: -

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Title: Physical Chemistry Chemical Physics
  Alternative Title : Phys. Chem. Chem. Phys.
Source Genre: Journal
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Pages: - Volume / Issue: 5 (23) Sequence Number: - Start / End Page: 5169 - 5175 Identifier: -