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  Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy

Liebermann, T., & Knoll, W. (2003). Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy. Langmuir, 19(5), 1567-1572.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-626B-A Version Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-626C-8
Genre: Journal Article

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 Creators:
Liebermann, T.1, Author           
Knoll, Wolfgang1, Author           
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

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 Abstract: We introduce surface-plasmon field-enhanced fluorescence microscopy for the on-line determination of hybridization reactions between targets from solution to complementary probe oligonucleotides which are surface-bound to a sensor substrate in a matrix format. This way, the parallel and real-time detection of the association and dissociation kinetics (rate constants) of hybrids of different mismatch situations can be quantitatively analyzed.

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Language(s): eng - English
 Dates: 2003-03-04
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 28399
ISI: 000181309600017
Other: P-03-288
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Title: Langmuir
  Alternative Title : Langmuir
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 19 (5) Sequence Number: - Start / End Page: 1567 - 1572 Identifier: ISSN: 0743-7463