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  Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation

Tameling, W. I., Vossen, J. H., Albrecht, M., Lengauer, T., Berden, J. A., Haring, M. A., et al. (2006). Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation. Plant Physiology, 140, 1233-1245.

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Tameling, Wladimir I., Autor
Vossen, Jack H., Autor
Albrecht, Mario1, Autor           
Lengauer, Thomas1, Autor           
Berden, Jan A., Autor
Haring, Michel A., Autor
Cornelissen, Ben J.C., Autor
Takken, Frank L. W.2, Autor
Affiliations:
1Computational Biology and Applied Algorithmics, MPI for Informatics, Max Planck Society, ou_40046              
2Max Planck Society, ou_persistent13              

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 Zusammenfassung: Resistance (R) proteins in plants confer specificity to the innate immune system. Most R proteins have a centrally located NB-ARC (nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4) domain. For two tomato (Lycopersicon esculentum) R proteins, I-2 and Mi-1, we have previously shown that this domain acts as an ATPase module that can hydrolyze ATP in vitro. To investigate the role of nucleotide binding and hydrolysis for the function of I-2 in planta, specific mutations were introduced in conserved motifs of the NB-ARC domain. Two mutations resulted in autoactivating proteins that induce a pathogen-independent hypersensitive response upon expression in planta. These mutant forms of I-2 were found to be impaired in ATP hydrolysis, but not in ATP binding, suggesting that the ATP- rather than the ADP-bound state of I-2 is the active form that triggers defense signaling. In addition, upon ADP binding, the protein displayed an increased affinity for ADP suggestive of a change of conformation. Based on these data, we propose that the NB-ARC domain of I-2, and likely of related R proteins, functions as a molecular switch whose state (on/off) depends on the nucleotide bound (ATP/ADP).

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Sprache(n): eng - English
 Datum: 2007-02-222006
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 314423
Anderer: Local-ID: C125673F004B2D7B-6189458FCBA1325CC125713A00666DDC-Albrecht2006b
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Titel: Plant Physiology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 140 Artikelnummer: - Start- / Endseite: 1233 - 1245 Identifikator: -