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キーワード:
Nicotiana; tobacco; tobacco alkaloids; nicotine; phenolics; conjugated polyamines; diterpene glycosides; liquid chromatography; mass spectrometry; jasmonic acid
Performance liquid-chromatography; basic compounds; accumulation; acid; induction; cultures; defense; tabacum; plants; biosynthesis
要旨:
A rapid, HPLC-based screening procedure for the main classes of secondary metabolites in Nicotiana attenuata leaves (alkaloids, phenolics, and diterpene glycosides) is reported. In a single step, leaves are extracted in aqueous acidified (0.5% acetic ac