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  Ultrastructural analysis of cytoplasmic intermediate filaments and the nuclear lamina in the mouse plasmacytoma cell line MPC-11 after the induction of vimentin synthesis

Wang, X., Willingale−Theune, J., Shoeman, R. L., Giese, G., & Traub, P. (1989). Ultrastructural analysis of cytoplasmic intermediate filaments and the nuclear lamina in the mouse plasmacytoma cell line MPC-11 after the induction of vimentin synthesis. European Journal of Cell Biology: EJCB, 50(2), 462-474. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2627942.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0000-84FB-0 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0000-84FC-F
資料種別: 学術論文

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EurJCellBiol_50_1989_462.pdf (全文テキスト(全般)), 10MB
 
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EurJCellBiol_50_1989_462.pdf
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 作成者:
Wang, Xiao, 著者
Willingale−Theune, Julia, 著者
Shoeman, Robert L.1, 2, 3, 著者           
Giese, Günter4, 5, 著者           
Traub, Peter, 著者
所属:
1Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
3Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              
4Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
5Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society, ou_1497720              

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 要旨: We examined cytoplasmic intermediate filaments (IFs) and the nuclear lamina in cells of the mouse plasmacytoma cell line MPC-11 (lacking both IF proteins and lamins A and C) after induction of vimentin synthesis with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) by means of whole-mount immunogold electron microscopy (IEM). The technique of IEM was modified to allow analysis of the cytoskeleton and nuclear lamina of cells grown in suspension culture employing antibodies against vimentin and lamin B. IEM showed that newly synthesized vimentin assembled into IFs which formed anastomosing networks throughout the cytoplasm, radiating primarily from the nucleus. The filaments decorated by gold-conjugated antibodies appeared to make contact with the lipid-depleted nuclear envelope residue either by directly terminating on it or through an indirect link via short fibers of varying diameter. Some filaments terminated on the subunits of the nuclear pore complexes but they did not pass through the pores. In the absence of lamins A and C, lamin B formed a nuclear lamina consisting of a globular-filamentous network anchoring the nuclear pore complexes.

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言語: eng - English
 日付: 1989-07-131989-09-261989-12
 出版の状態: 出版
 ページ: -
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 査読: 査読あり
 識別子(DOI, ISBNなど): URI: http://www.ncbi.nlm.nih.gov/pubmed/2627942
 学位: -

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出版物 1

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出版物名: European Journal of Cell Biology : EJCB
  その他 : Eur. J. Cell Biol.
種別: 学術雑誌
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出版社, 出版地: Stuttgart : Wissenschaftliche Verlagsgesellschaft.
ページ: - 巻号: 50 (2) 通巻号: - 開始・終了ページ: 462 - 474 識別子(ISBN, ISSN, DOIなど): ISSN: 0070-2463
CoNE: https://pure.mpg.de/cone/journals/resource/954925486755