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キーワード:
Drosophila, Oogenesis, Germline stem cell, Stem cell niche, Cell fate, Steroid, miR-125, Tom, Notch, Delta, Notch cell reprogramming
要旨:
Stem cell niches act as signaling platforms that regulate stem cell self-renewal and sustain stem cells throughout life; however, the specific developmental events controlling their assembly are not well understood. Here we show that during Drosophila ovarian germline stem cell niche formation, the status of Notch signaling in the cell can be reprogrammed. This is controlled via steroid-induced miR-125, which targets a negative regulator of Notch signaling, Tom. Thus, miR-125 acts as a spatiotemporal coordinator between paracrine Notch and endocrine steroid signaling. Moreover, a dual security mechanism for Notch signaling activation exists to ensure the robustness of niche assembly. Particularly, stem cell niche cells can be specified either via lateral inhibition, in which a niche cell precursor acquires Notch signal-sending status randomly, or via peripheral induction, whereby Delta is produced by a specific cell. When one mechanism is perturbed due to mutations, developmental defects, or environmental stress, the remaining mechanism ensures that the niche is formed, perhaps abnormal but still functional. This guarantees that the germline stem cells will have their residence, thereby securing progressive oogenesis, thus, organism reproduction.
