Cellular resolution circuit mapping with temporal-focused excitation of 
soma-targeted channelrhodopsin

Baker, Christopher A.; Elyada, Yishai M.; Parra, Andres; Bolton, M. McLean

doi:10.7554/eLife.14193

Lokale TagsFreigabegeschichteDetailsÜbersicht

Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin

Baker, C. A., Elyada, Y. M., Parra, A., & Bolton, M. M. (2016). Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin. eLife, 5, e14193-e14193. doi:10.7554/eLife.14193.

Item is Freigegeben

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:

Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2ADA-9 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2AF8-5

Genre: Zeitschriftenartikel

Alternativer Titel : eLife

ausblenden:

Urheber:
Baker, Christopher A.¹, Autor
Elyada, Yishai M.¹, Autor
Parra, Andres¹, Autor
Bolton, M. McLean¹, Autor

Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288

Inhalt

einblenden:

ausblenden:

Schlagwörter: circuit, connectivity, mouse, Neuroscience, Optogenetics

Zusammenfassung: We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.