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  Double-flow focused liquid injector for efficient serial femtosecond crystallography

Oberthuer, D., Knoška, J., Wiedorn, M. O., Beyerlein, K. R., Bushnell, D. A., Kovaleva, E. G., Heymann, M., Gumprecht, L., Kirian, R. A., Barty, A., Mariani, V., Tolstikova, A., Adriano, L., Awel, S., Barthelmess, M., Dörner, K., Paulraj, L. X., Yefanov, O., James, D. R., Nelson, G., Wang, D., Calvey, G., Chen, Y., Schmidt, A., Szczepek, M., Frielingsdorf, S., Lenz, O., Snell, E., Robinson, P. J., Šarler, B., Belšak, G., Maček, M., Wilde, F., Aquila, A., Boutet, S., Liang, M., Hunter, M. S., Scheerer, P., Lipscomb, J. D., Weierstall, U., Kornberg, R. D., Spence, J. C. H., Pollack, L., Chapman, H. N., & Bajt, S. (2017). Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7:. doi:10.1038/srep44628.

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資料種別: 学術論文

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srep44628.pdf (出版社版), 2MB
ファイルのパーマリンク:
https://hdl.handle.net/11858/00-001M-0000-002D-549A-E
ファイル名:
srep44628.pdf
説明:
-
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閲覧制限:
公開
MIMEタイプ / チェックサム:
application/pdf / [MD5]
技術的なメタデータ:
著作権日付:
2017
著作権情報:
© The Author(s)
CCライセンス:
-

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URL:
https://doi.org/10.1038/srep44628 (出版社版)
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-
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作成者

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 作成者:
Oberthuer, Dominik1, 著者
Knoška, Juraj1, 2, 著者
Wiedorn, Max O.1, 2, 著者
Beyerlein, Kenneth R.1, 著者
Bushnell, David A.3, 著者
Kovaleva, Elena G.4, 著者
Heymann, Michael1, 著者
Gumprecht, Lars1, 著者
Kirian, Richard A.5, 著者
Barty, Anton1, 著者
Mariani, Valerio1, 著者
Tolstikova, Aleksandra1, 2, 著者
Adriano, Luigi6, 著者
Awel, Salah1, 7, 著者
Barthelmess, Miriam1, 著者
Dörner, Katerina1, 著者
Paulraj, Lourdu Xavier1, 2, 8, 著者           
Yefanov, Oleksandr1, 著者
James, Daniel R.5, 著者
Nelson, Garrett5, 著者
Wang, Dingjie5, 著者Calvey, George9, 著者Chen, Yujie9, 著者Schmidt, Andrea10, 著者Szczepek, Michael10, 著者Frielingsdorf, Stefan11, 著者Lenz, Oliver11, 著者Snell, Edward12, 著者Robinson, Philip J.4, 著者Šarler, Božidar13, 14, 著者Belšak, Grega14, 著者Maček, Marjan14, 著者Wilde, Fabian15, 著者Aquila, Andrew16, 著者Boutet, Sébastien16, 著者Liang, Mengning16, 著者Hunter, Mark S.16, 著者Scheerer, Patrick10, 著者Lipscomb, John D.17, 著者Weierstall, Uwe5, 著者Kornberg, Roger D.3, 著者Spence, John C. H.5, 著者Pollack, Lois9, 著者Chapman, Henry N.1, 2, 17, 著者Bajt, Saša6, 著者 全て表示
所属:
1Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany, ou_persistent22              
2Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              
3Department of Structural Biology, Stanford University, School of Medicine, Stanford, California 94305, USA, ou_persistent22              
4SSRL, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA, ou_persistent22              
5Department of Physics, Arizona State University, Tempe, AZ, USA, ou_persistent22              
6Photon Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany, ou_persistent22              
7Centre for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              
8International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
9School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA, ou_persistent22              
10Institut für Medizinische Physik und Biophysik, Group Protein X-ray Crystallography and Signal Transduction, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany, ou_persistent22              
11Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Straße des 17, Juni 135, 10623 Berlin, Germany, ou_persistent22              
12Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA, ou_persistent22              
13Laboratory for Multiphase Processes, University of Nova Gorica, Vipavska 13, SI-5000 Nova Gorica, Slovenia, ou_persistent22              
14Laboratory for Simulation of Materials and Processes, Institute of Metals and Technology, Lepi pot 11, SI-1000 Ljubljana, Slovenia, ou_persistent22              
15Helmholtz-Zentrum Geesthacht, Max-Planck-Straße 1, 21502 Geesthacht, Germany, ou_persistent22              
16LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA, ou_persistent22              
17Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA, ou_persistent22              

内容説明

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キーワード: Fluid dynamics, Nanocrystallography
 要旨: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

資料詳細

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言語: eng - English
 日付: 2017-01-062017-02-102017-03-16
 出版の状態: オンラインで出版済み
 ページ: 10
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): DOI: 10.1038/srep44628
 学位: -

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出版物 1

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出版物名: Scientific Reports
  省略形 : Sci. Rep.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: London, UK : Nature Publishing Group
ページ: - 巻号: 7 通巻号: 44628 (2017) 開始・終了ページ: - 識別子(ISBN, ISSN, DOIなど): ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322