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  Balance of ATPase stimulation and nucleotide exchange is not required for efficient refolding activity of the DnaK chaperone

Groemping, Y., Seidel, R., & Reinstein, J. (2005). Balance of ATPase stimulation and nucleotide exchange is not required for efficient refolding activity of the DnaK chaperone. FEBS Letters, 579(25), 5713-5717. doi:10.1016/j.febslet.2005.09.056.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-701A-B Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-701B-9
Genre: Journal Article
Alternative Title : Balance of ATPase stimulation and nucleotide exchange is not required for efficient refolding activity of the DnaK chaperone

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FEBSLett_579_2005_5713.pdf (Any fulltext), 112KB
 
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 Creators:
Groemping, Yvonne1, Author           
Seidel, Ralf, Author
Reinstein, Jochen1, Author           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: Chaperone; Folding; Kinetics; DnaK-system; Regulation
 Abstract: The DnaK system from Thermus thermophilus (DnaK(Tth)) exhibits pronounced differences in organisation and regulation to its mesophile counterpart from Escherichia coli (DnaK(Eco)). While the ATPase cycle of DnaK(Eco) is tightly regulated by the concerted action of the two cofactors DnaJ(Eco) and GrpE(Eco), the DnaK(Tth) system features an imbalance in this cochaperone mediated regulation. GrpE(Tth) considerably accelerates the ATP/ADP exchange, but DnaJ(Tth) only slightly stimulates ATPase activity, believed to be a key step for chaperone activity of DnaK(Eco). By in vitro complementation assays, we could not detect significant ATPase-stimulation of orthologous DnaJ(Tth) . DnaKEco or DnaJ(Eco). DnaK(Tth)-complexes as compared to the DnaK(Eco) system, although they were nevertheless active in luciferase refolding experiments. Assistance of protein recovery by DnaK thus seems to be uncoupled of the magnitude of DnaJ mediated ATPase-stimulation.

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Language(s): eng - English
 Dates: 2005-09-222005-07-282005-09-222005-10-052005-10-24
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665519
DOI: 10.1016/j.febslet.2005.09.056
URI: http://www.ncbi.nlm.nih.gov/pubmed/16225874
URI: http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2005.09.056/abstract
URI: http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2005.09.056/full
Other: 6471
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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 579 (25) Sequence Number: - Start / End Page: 5713 - 5717 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501