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  Miniaturization of two-photon microscopy for imaging in freely moving animals

Helmchen, F., Denk, W., & Kerr, J. N. (2013). Miniaturization of two-photon microscopy for imaging in freely moving animals. Cold Spring Harbor Protocols, 2013(10), 904-913. doi:10.1101/pdb.top078147.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-C7A6-1 Versions-Permalink: https://hdl.handle.net/21.11116/0000-0008-CFA4-6
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 Urheber:
Helmchen, Fritjof1, Autor           
Denk, Winfried2, Autor           
Kerr, Jason ND1, Autor           
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Zusammenfassung: This article describes the development and application of miniaturized two-photon-excited fluorescence microscopes (“two-photon fiberscopes”). Two-photon fiberscopes have been developed with the aim of enabling high-resolution imaging of neural activity in freely behaving animals. They use fiber optics to deliver laser light for two-photon excitation. Their small front piece typically contains a miniature scanning mechanism and imaging optics. Two-photon fiberscopes can be made sufficiently small and lightweight to be carried by rats and mice and to allow virtually unrestricted movement within a behavioral arena. Typically mounted to the animal’s skull above a cranial window, two-photon fiberscopes permit imaging of cells down to at least 250 µm below the brain surface (e.g., in rat neocortex). In freely exploring animals, action-potential-evoked calcium transients can be imaged in individual somata of visual cortex neurons bulk-labeled with a calcium indicator. Two-photon fiberscopes thus enable high-resolution optical recording of neural activity with cellular resolution during natural behaviors

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Sprache(n): eng - English
 Datum: 2013
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: Anderer: 8057
DOI: 10.1101/pdb.top078147
URI: http://cshprotocols.cshlp.org/content/2013/10/pdb.top078147.full
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Titel: Cold Spring Harbor Protocols
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: NY : Cold Spring Harbor Laboratory Press
Seiten: - Band / Heft: 2013 (10) Artikelnummer: - Start- / Endseite: 904 - 913 Identifikator: ISSN: 1940-3402