Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Quantification of total calcium in terminal cisternae of skinned muscle fibres by imaging electron energy-loss spectroscopy

Stegmann, H., Wepf, R., Schröder, R. R., & Fink, R. H. A. (1999). Quantification of total calcium in terminal cisternae of skinned muscle fibres by imaging electron energy-loss spectroscopy. Journal of Muscle Research and Cell Motility, 20, 505-515. doi:10.1023/A:1005522912044.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-59EE-8 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-59F3-7
Genre: Zeitschriftenartikel
Alternativer Titel : Quantification of total calcium in terminal cisternae of skinned muscle fibres by imaging electron energy-loss spectroscopy

Dateien

einblenden: Dateien
ausblenden: Dateien
:
JMuscleResCellMotil_20_1999_505.pdf (beliebiger Volltext), 220KB
 
Datei-Permalink:
-
Name:
JMuscleResCellMotil_20_1999_505.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
http://link.springer.com/content/pdf/10.1023%2FA%3A1005522912044.pdf (beliebiger Volltext)
Beschreibung:
-
OA-Status:
externe Referenz:
http://dx.doi.org/10.1023/A:1005522912044 (beliebiger Volltext)
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Stegmann, Heiko1, Autor           
Wepf, Roger, Autor
Schröder, Rasmus R.1, 2, Autor           
Fink, Rainer H. A., Autor
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Skinned muscle fibers are ideal model preparations for the investigation of Ca2+−regulatory mechanisms. Their internal ionic milieu can be easily controlled and distinct physiological states are well defined. We have measured the total Ca content in the terminal cisternae of such preparations using imaging electron energy−loss spectroscopy (Image−EELS) as a new approach for quantification of sub−cellular element distributions. Murine muscle fibers submitted to a standardized calcium−loading procedure were cryo−fixed with a combined solution exchanger/plunge freezing device. Energy−filtered image series were recorded from ultrathin freeze−dried cryosections of samples immobilized in either relaxed or caffeine−contracted state. From these image series, electron energy−loss spectra were extracted by digital image−processing and quantitatively processed by multiple−least−squares−fitting with reference spectra. The calculated fit coefficients were converted to Ca−concentrations by a calibration obtained from Ca−standards. Total Ca−contents in the terminal cisternae of skinned skeletal muscle fibers decreased upon caffeine−induced Ca−release from 123 ± 159 (±11) to 73 ± 102 (±8) mmol/kg d.w. (weighted mean ± SD (±SEM))

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 1999-05-031999-04-301999-05-031999
 Publikationsstatus: Erschienen
 Seiten: 11
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 666532
DOI: 10.1023/A:1005522912044
URI: http://www.ncbi.nlm.nih.gov/pubmed/10555069
Anderer: 4477
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of Muscle Research and Cell Motility
  Andere : J. Muscle Res. Cell Motil.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: London : Chapman and Hall
Seiten: - Band / Heft: 20 Artikelnummer: - Start- / Endseite: 505 - 515 Identifikator: ISSN: 0142-4319
CoNE: https://pure.mpg.de/cone/journals/resource/954925472360