In vivo protein crystallization opens new routes in structural biology

Koopmann, Rudolf; Cupelli, Karolina; Redecke, Lars; Nass, Karol; DePonte, Daniel P.; White, Thomas A.; Stellato, Francesco; Rehders, Dirk; Liang, Mengning; Andreasson, Jakob; Aquila, Andrew; Bajt, Saša; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J.; Bostedt, Christoph; Boutet, Sébastien; Bozek, John D.; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; Doak, R. Bruce; Ekeberg, Tomas; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Holger; Foucar, Lutz; Graafsma, Heinz; Gumprecht, Lars; Hajdu, Janos; Hampton, Christina. Y; Hartmann, Andreas; Hartmann, Robert; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Hunter, Mark S.; Kassemeyer, Stephan; Kirian, Richard A.; Lomb, Lukas; Maia, Filipe R. N. C.; Kimmel, Nils; Martin, Andrew V.; Messerschmidt, Marc; Reich, Christian; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilme; Schulz, Joachim; Seibert, M. Marvin; Shoeman, Robert L.; Sierra, Raymond G.; Soltau, Heike; Stern, Stephan; Strüder, Lothar; Timneanu, Nicusor; Ullrich, Joachim; Wang, Xiaoyu; Weidenspointner, Georg; Weierstall, Uwe; Williams, Garth J.; Wunderer, Cornelia B.; Fromme, Petra; Spence, John C. H.; Stehle, Thilo; Chapman, Henry N.; Betzel, Christian; Duszenko, Michael

doi:10.1038/nmeth.1859

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In vivo protein crystallization opens new routes in structural biology

Koopmann, R., Cupelli, K., Redecke, L., Nass, K., DePonte, D. P., White, T. A., et al. (2012). In vivo protein crystallization opens new routes in structural biology. Nature methods, 9(3), 259-262. doi:10.1038/nmeth.1859.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-1E14-3 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-1E15-1

Genre: Journal Article

Alternative Title : In vivo protein crystallization opens new routes in structural biology

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Koopmann, Rudolf, Author
Cupelli, Karolina, Author
Redecke, Lars, Author
Nass, Karol¹, Author
DePonte, Daniel P., Author
White, Thomas A., Author
Stellato, Francesco, Author
Rehders, Dirk, Author
Liang, Mengning, Author
Andreasson, Jakob, Author
Aquila, Andrew, Author
Bajt, Saša, Author
Barthelmess, Miriam, Author
Barty, Anton, Author
Bogan, Michael J., Author
Bostedt, Christoph, Author
Boutet, Sébastien, Author
Bozek, John D., Author
Caleman, Carl, Author
Coppola, Nicola, Author
Davidsson, Jan, AuthorDoak, R. Bruce¹, Author Ekeberg, Tomas, AuthorEpp, Sascha W., AuthorErk, Benjamin¹, Author Fleckenstein, Holger, AuthorFoucar, Lutz¹, Author Graafsma, Heinz, AuthorGumprecht, Lars, AuthorHajdu, Janos, AuthorHampton, Christina. Y, AuthorHartmann, Andreas, AuthorHartmann, Robert, AuthorHauser, Günter, AuthorHirsemann, Helmut, AuthorHoll, Peter, AuthorHunter, Mark S., AuthorKassemeyer, Stephan¹, Author Kirian, Richard A., AuthorLomb, Lukas¹, Author Maia, Filipe R. N. C., AuthorKimmel, Nils, AuthorMartin, Andrew V., AuthorMesserschmidt, Marc, AuthorReich, Christian, AuthorRolles, Daniel¹, Author Rudek, Benedikt¹, Author Rudenko, Artem, AuthorSchlichting, Ilme¹, Author Schulz, Joachim, AuthorSeibert, M. Marvin, AuthorShoeman, Robert L.¹, Author Sierra, Raymond G., AuthorSoltau, Heike, AuthorStern, Stephan, AuthorStrüder, Lothar, AuthorTimneanu, Nicusor, AuthorUllrich, Joachim, AuthorWang, Xiaoyu, AuthorWeidenspointner, Georg, AuthorWeierstall, Uwe, AuthorWilliams, Garth J., AuthorWunderer, Cornelia B., AuthorFromme, Petra, AuthorSpence, John C. H., AuthorStehle, Thilo, AuthorChapman, Henry N., AuthorBetzel, Christian, AuthorDuszenko, Michael, Author more..

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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700

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Abstract: Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X−ray crystallographic analysis. We prepared nano−sized in vivo−grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free−electron laser−based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology

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Language(s): eng - English

Dates: Submitted: 2011-05-02Accepted: 2011-12-21Date issued: 2012-01-29

Publication Status: Issued

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Rev. Type: Peer

Identifiers: eDoc: 664425
DOI: 10.1038/nmeth.1859
URI: http://www.ncbi.nlm.nih.gov/pubmed/22286384
URI: http://www.nature.com/nmeth/journal/v9/n3/full/nmeth.1859.html
URI: http://www.nature.com/nmeth/journal/v9/n3/pdf/nmeth.1859.pdf
Other: 7761

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Title: Nature methods

Other : Nature methods

Source Genre: Journal

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Publ. Info: New York, NY : Nature Pub. Group

Pages: - Volume / Issue: 9 (3) Sequence Number: - Start / End Page: 259 - 262 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556