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キーワード:
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要旨:
One purpose of the biomedical literature is to report results
in sufficient detail that the methods of data collection and
analysis can be independently replicated and verified. Here we
present reporting guidelines for gene expression localization
experiments: the minimum information specification for
in
situ
hybridization and immunohistochemistry experiments
(MISFISHIE). MISFISHIE is modeled after the Minimum
Information About a Microarray Experiment (MIAME)
specification for microarray experiments. Both guidelines define
what information should be reported without dictating a format
for encoding that information. MISFISHIE describes six types
of information to be provided for each experiment: experimental
design, biomaterials and treatments, reporters, staining,
imaging data and image characterizations. This specification
has benefited the consortium within which it was developed
and is expected to benefit the wider research community.
We welcome feedback from the scientific community to help
improve our proposal.
