Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide

Ylera, Francisco; Lurz, Rudi; Erdmann, Volker A.; Fürste, Jens Peter

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Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide

Ylera, F., Lurz, R., Erdmann, V. A., & Fürste, J. P. (2002). Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide. Biochemical and Biophysical Research Communications, 290(5), 1583-1588.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8C4C-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8C4D-0

Genre: Journal Article

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Creators:
Ylera, Francisco, Author
Lurz, Rudi¹, Author
Erdmann, Volker A., Author
Fürste, Jens Peter, Author

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1Max Planck Society, ou_persistent13

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Free keywords: in vitro selection; SELEX; electron microscopy; gold labeling; high-affinity RNA; aptamer; amyloid; Alzheimer's disease

Abstract: Alzheimer's disease is correlated with the deposition of amyloid peptides in the brain of the patients. The amyloid is thus a major target in the search for novel diagnostic and therapeutic approaches. The present work employs in vitro selection to develop new tools for the study of the Alzheimer's disease. The selection strategy enables the design of specific nucleic acids (aptamers) against virtually any target molecule. High-affinity RNA aptamers against the A4(1–40) were isolated from a combinatorial library of ~1015 different molecules. The apparent dissociation constants Kd of these aptamers are 29–48 nM. The binding of the RNA to the amyloid fibrils was confirmed by electron microscopy. The chemical synthesis of these nucleic acids enables tailor-made modifications. By introduction of specific reporter groups these RNAs can become suitable tools for analytical and diagnostic purposes. Thus, this study may introduce a new approach for diagnosis of the Alzheimer's disease.

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Language(s): eng - English

Dates: Date issued: 2002-02-22

Publication Status: Issued

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Identifiers: eDoc: 24242

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Title: Biochemical and Biophysical Research Communications

Source Genre: Journal

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Pages: - Volume / Issue: 290 (5) Sequence Number: - Start / End Page: 1583 - 1588 Identifier: -