Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Is the in situ accessibility of the 16S rRNA of Escherichia coli for Cy3-labeled oligonucleotide probes predicted by a three-dimensional structure model of the 30S ribosomal subunit?

Behrens, S., Fuchs, B. M., Mueller, F., & Amann, R. (2003). Is the in situ accessibility of the 16S rRNA of Escherichia coli for Cy3-labeled oligonucleotide probes predicted by a three-dimensional structure model of the 30S ribosomal subunit? Applied and Environmental Microbiology, 69(8), 4935-4941. doi:10.1128/AEM.69.8.4935-4941.2003.

Item is Freigegeben

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:

Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-89E2-B Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-89E3-9

Genre: Zeitschriftenartikel

Alternativer Titel : Appl. Environ. Microbiol.

Dateien

einblenden: Dateien

Externe Referenzen

Urheber

ausblenden:

Urheber:
Behrens, Sebastian¹, Autor
Fuchs, Bernhard M.¹, Autor
Mueller, Florian¹, Autor
Amann, Rudolf¹, Autor

Affiliations:
1Max Planck Society, ou_persistent13

Inhalt

ausblenden:

Schlagwörter: -

Zusammenfassung: Systematic studies on the hybridization of fluorescently labeled, rRNA-targeted oligonucleotides have shown strong variations in in situ accessibility. Reliable predictions of target site accessibility would contribute to more-rational design of probes for the identification of individual microbial cells in their natural environments. During the past 3 years, numerous studies of the higher-order structure of the ribosome have advanced our understanding of its spatial conformation. These studies range from the identification of rRNA-rRNA interactions based on covariation analyses to physical imaging of the ribosome for the identification of protein-rRNA interactions. Here we reevaluate our Escherichia coli 16S rRNA in situ accessibility data with regard to a tertiary-structure model of the small subunit of the ribosome. We localized target sequences of 176 oligonucleotides on a 3.0-Å-resolution three-dimensional (3D) model of the 30S ribosomal subunit. Little correlation was found between probe hybridization efficiency and the proximity of the probe target region to the surface of the 30S ribosomal subunit model. We attribute this to the fact that fluorescence in situ hybridization is performed on fixed cells containing denatured ribosomes, whereas 3D models of the ribosome are based on its native conformation. The effects of different fixation and hybridization protocols on the fluorescence signals conferred by a set of 10 representative probes were tested. The presence or absence of the strongly denaturing detergent sodium dodecyl sulfate had a much more pronounced effect than a change of fixative from paraformaldehyde to ethanol.

Details

ausblenden:

Sprache(n): eng - English

Datum: Erschienen: 2003-08

Publikationsstatus: Erschienen

Seiten: -

Ort, Verlag, Ausgabe: -

Inhaltsverzeichnis: -

Art der Begutachtung: -

Identifikatoren: eDoc: 194819
ISI: 000184672500076
DOI: 10.1128/AEM.69.8.4935-4941.2003

Art des Abschluß: -

Veranstaltung

Entscheidung

Projektinformation

Quelle 1

ausblenden:

Titel: Applied and Environmental Microbiology

Alternativer Titel : Appl. Environ. Microbiol.

Genre der Quelle: Zeitschrift

Urheber:

Affiliations:

Ort, Verlag, Ausgabe: -

Seiten: - Band / Heft: 69 (8) Artikelnummer: - Start- / Endseite: 4935 - 4941 Identifikator: ISSN: 0099-2240