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  Yersinia enterocolitica type III secretion depends on the proton motive force but not on the flagellar motor components MotA and MotB

Wilharm, G., Lehmann, V., Krauss, K., Lehnert, B., Richter, S., Ruckdeschel, K., et al. (2004). Yersinia enterocolitica type III secretion depends on the proton motive force but not on the flagellar motor components MotA and MotB. Infection and Immunity, 72(7), 4004-4009. doi:10.1128/IAI.72.7.4004-4009.2004.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-882A-4 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-882B-2
Genre: Zeitschriftenartikel
Alternativer Titel : Infect Immun

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 Urheber:
Wilharm, Gottfried, Autor
Lehmann, Verena1, Autor
Krauss, Kristina, Autor
Lehnert, Beatrix, Autor
Richter, Susanna, Autor
Ruckdeschel, Klaus, Autor
Heesemann, Jürgen, Autor
Trülzsch, Konrad, Autor
Affiliations:
1Max Planck Society, ou_persistent13              

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Schlagwörter: -
 Zusammenfassung: The flagellum is believed to be the common ancestor of all type III secretion systems (TTSSs). In Yersinia enterocolitica, expression of the flagellar TTSS and the Ysc (Yop secretion) TTSS are inversely regulated. We therefore hypothesized that the Ysc TTSS may adopt flagellar motor components in order to use the pathogenicity-related translocon in a drill-like manner. As a prerequisite for this hypothesis, we first tested a requirement for the proton motive force by both systems using the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP). Motility as well as type III-dependent secretion of Yop proteins was inhibited by CCCP. We deleted motAB, which resulted in an immotile phenotype. This mutant, however, secreted amounts of Yops to the supernatant comparable to those of the wild type. Translocation of Yops into host cells was also not affected by the motAB deletion. Virulence of the mutant was comparable to that of the wild type in the mouse oral infection model. Thus, the hypothesis that the Ysc TTSS might adopt flagellar motor components was not confirmed. The finding that, in addition to consumption of ATP, Ysc TTSS requires the proton motive force is discussed.

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Sprache(n): eng - English
 Datum: 2004-07
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 230844
DOI: 10.1128/IAI.72.7.4004-4009.2004
 Art des Abschluß: -

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Titel: Infection and Immunity
  Alternativer Titel : Infect Immun
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 72 (7) Artikelnummer: - Start- / Endseite: 4004 - 4009 Identifikator: ISSN: 1098-5522