Gene expression analysis of pancreatic cell lines reveals genes overexpressed 
in pancreatic cancer

Aldinger, Ingo; Dittert, Dag; Peiper, Matthias; Fusco, Alberto; Chiappetta, Gennaro; Staub, Eike; Loehr, Matthias; Jesnowsk, Ralf; Baretton, Gustavo; Ockert, Detlef; Saeger, Hans-Detlev; Gruetzmann, Robert; Pilarsky, Christian

doi:10.1159/000086537

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Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer

Aldinger, I., Dittert, D., Peiper, M., Fusco, A., Chiappetta, G., Staub, E., et al. (2005). Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. Pancreatology: Official Journal of the International Association of Pancreatology (IAP), European Pancreatic Club, 5(4-5), 370-379. doi:10.1159/000086537.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8614-2 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8615-F

Genre: Journal Article

Alternative Title : EPC

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Creators:
Aldinger, Ingo, Author
Dittert, Dag, Author
Peiper, Matthias, Author
Fusco, Alberto, Author
Chiappetta, Gennaro, Author
Staub, Eike¹, Author
Loehr, Matthias, Author
Jesnowsk, Ralf, Author
Baretton, Gustavo, Author
Ockert, Detlef, Author
Saeger, Hans-Detlev, Author
Gruetzmann, Robert, Author
Pilarsky, Christian, Author

Affiliations:
1Max Planck Society, ou_persistent13

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Free keywords: Pancreas Cancer Cell line Primary isolates Microarray Thymosin Immunohistochemistry

Abstract: Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin beta-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatic tissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.

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Language(s): eng - English

Dates: Date issued: 2005-06-23

Publication Status: Issued

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Identifiers: eDoc: 264875
DOI: 10.1159/000086537

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Title: Pancreatology : Official Journal of the International Association of Pancreatology (IAP), European Pancreatic Club

Alternative Title : EPC

Source Genre: Journal

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Pages: - Volume / Issue: 5 (4-5) Sequence Number: - Start / End Page: 370 - 379 Identifier: ISSN: 1424-3903