非表示:
キーワード:
Animals; Antigens, CD/*metabolism; Antigens, CD24/*metabolism; Cell Differentiation; Cells, Cultured; Ependyma/*cytology/metabolism; Flow Cytometry; *Gene Expression; Glycoproteins/*metabolism; Immunohistochemistry; Lateral Ventricles/*cytology/metabolism; Mice; Neural Stem Cells/cytology/metabolism; Oligonucleotide Array Sequence Analysis; Peptides/*metabolism; Reverse Transcriptase Polymerase Chain Reaction; Spinal Cord/*cytology/metabolism
要旨:
In contrast to ependymal cells located above the subventricular zone (SVZ) of the adult lateral ventricle wall (LVW), adult spinal cord (SC) ependymal cells possess certain neural stem cell characteristics. The molecular basis of this difference is unknown. In this study, antibodies against multiple cell surface markers were applied to isolate pure populations of SC and LVW ependymal cells, which allowed a direct comparison of their in vitro behavior and in vivo gene expression profile. Isolated CD133(+)/CD24(+)/CD45(-)/CD34(-) ependymal cells from the SC displayed in vitro self-renewal and differentiation capacity, whereas those from the LVW did not. SC ependymal cells showed a higher expression of several genes involved in cell division, cell cycle regulation, and chromosome stability, which is consistent with a long-term self-renewal capacity, and shared certain transcripts with neural stem cells of the embryonic forebrain. They also expressed several retinoic acid (RA)-regulated genes and responded to RA exposure. LVW ependymal cells showed higher transcript levels of many genes regulated by transforming growth factor-beta family members. Among them were Dlx2, Id2, Hey1, which together with Foxg1 could explain their potential to turn into neuroblasts under certain environmental conditions.
