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キーワード:
phage display; cdna library; orf selection; phagemid; protein-protein interaction; next generation sequencing (ngs); open reading frames; antibody variable domains; fd adsorption protein; filamentous phage; escherichia-coli; surface expression; functional cloning; bacteriophage fd; helper phage; selection
要旨:
The last decade has seen a steady increase in screening of cDNA expression product libraries displayed on the surface of filamentous bacteriophage. At the same time, the range of applications extended from the identification of novel allergens over disease markers to protein-protein interaction studies. However, the generation and selection of cDNA phage display libraries is subjected to intrinsic biological limitations due to their complex nature and heterogeneity, as well as technical difficulties regarding protein presentation on the phage surface. Here, we review the latest developments in this field, discuss a number of strategies and improvements anticipated to overcome these challenges making cDNA and open reading frame (ORF) libraries more readily accessible for phage display. Furthermore, future trends combining phage display with next generation sequencing (NGS) will be presented.
