非表示:
キーワード:
Animal; Cysteine Endopeptidases/ip [Isolation & Purification]; *Cysteine Endopeptidases; Microscopy, Electron; Multienzyme Complexes/ip [Isolation & Purification]; *Multienzyme Complexes; Muscles/en [Enzymology]; Rats; Staining and Labeling; Support, Non-U.S. Gov't
要旨:
One electron micrographs, negatively stained multicatalytic proteinase molecules are viewed end-on (ring shaped) or side-on (rectangular shaped). For aurothioglucose, ammonium molybdate- and phosphotungstate-stained molecules, the dimensions measured are consistent. In contrast, uranyl acetate-staining reveals ring-shaped particles which vary in diameter between 12 and 16 nm. This is due to a partial collapse and substantial flattening of the structure. Digital image analysis of side-on views of the particles reveals a tripartite, reel-shaped structure. Within the ring-like, end-on projections of ammonium molybdate-stained molecules six local centres of mass can be discerned; their position appears to depart, however, from a true six-fold symmetry.
